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. 2021 Jun 4;10(6):913. doi: 10.3390/antiox10060913

Figure 3.

Figure 3

Reduction of FurA by TrxA. FurA WT (1 µM) was incubated with 0.5 µM TrxA in the presence of 0.2 mM NADPH, 0.5 mM EDTA, and 5 nM NTR to assess its reduction by NTR-reduced TrxA. FurA was also incubated with 0.2 mM NADPH and 5 nM NRT to determine whether NADPH-reduced NTR affected its redox status with 1 mM DTT as a positive control for protein reduction. Proteins were then precipitated with 10% (w/v) TCA, incubated with 20 mM AMS, loaded on non-reducing 15% SDS–polyacrylamide gels, and stained with Coomassie.