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. 2021 Jun 1;10(6):492. doi: 10.3390/biology10060492

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) Bacteria encapsulation in alginate beads; bean seeds inoculated with B. subtilis encapsulated in alginate beads. (b) Bean plants growing in the greenhouse; products amplified by PCR with primers Bsub5F-Bsub3R (600 bp) on the template of DNA samples extracted from bean plants inoculated with B. subtilis (B1-B5) and DNA of B. subtilis and P. aeruginosa as control positive and negative, respectively. DNA molecular market: GeneRul-er-100bp-Plus. (c) The graph shows the mean area of the lesions at 7 days post-inoculation (dpi) (* p ˂ 0.05). Images show the course of infection in plant control in comparison with a plant germinated previously with B. subtilis along 5-, 6-, and 7-dpi (representative images of the lesions are shown on different leaves).