Table 2.
Primers used in this study.
| Primer | Sequence (5′ → 3′) | Product Size (bp) | Reference | Used for |
|---|---|---|---|---|
| 16SF | AGAGTTTGATCCTGGCTCAG | 1500 | [31] | 16S-rRNA partial amplification |
| 16SR | TACGGCTACCTTGTTACGA | 1500 | [31] | 16S-rRNA partial amplification |
| Bac_FWd | AGCAGTGGGGAATATTGGAC | 700 | This study | 16S-rRNA partial amplification |
| Bac_Rev1 | TCTAATCCTGTTTGCTCCCC | 700 | This study | 16S-rRNA partial amplification |
| Bsub5F | AAGTCGAGCGGACAGATGG | 600 | [33] | Species-specific primers for B. subtilis identification |
| Bsub3R | CCAGTTTCCAATGACCCTCCCC | 600 | [33] | Species-specific primers for B. subtilis identification |
| ITUCF | GGCTGCTGCAGATGCTTTAT | 423 | [34] | Detection of ituC gene (Iturin) |
| ITUCR | TCGCAGATAATCGCAGTGAG | 423 | [34] | Detection of ituC gene (Iturin) |
| FENDF | GGCCCGTTCTCTAAATCCAT | 270 | [34] | Detection of fenD gene (Fengycin) |
| FENDR | GTCATGCTGACGAGAGCAAA | 270 | [34] | Detection of fenD gene (Fengycin) |
| BACF | CAGCTCATGGGAATGCTTTT | 500 | [34] | Detection of bacA gene (Bacylisin) |
| BACR | CTCGGTCCTGAAGGGACAAG | 500 | [34] | Detection of bacA gene (Bacylisin) |
| SRFAF | TCGGGACAGGAAGACATCAT | 200 | [34] | Detection of sfrAA gene (Surfactin) |
| SRFAR | CCACTCAAACGGATAATCCTGA | 200 | [34] | Detection of sfrAA gene (Surfactin) |
| SPASF | GGTTTGTTGGATGGAGCTGT | 375 | [34] | Detection of spaS gene (Subtilin) |
| SPASR | GCAAGGAGTCAGAGCAAGGT | 375 | [34] | Detection of spaS gene (Subtilin) |
| BMYBF | GAATCCCGTTGTTCTCCAAA | 370 | [34] | Detection of bmyB gene (Bacillomycin) |
| BMYBR | GCGGGTATTGAATGCTTGTT | 370 | [34] | Detection of bmyB gene (Bacillomycin) |