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. 2021 May 28;13(6):808. doi: 10.3390/pharmaceutics13060808

Figure 1.

Figure 1

Impact of IFN-α 2a (1000 U/mL, black circles; 5000 U/mL, black triangles) on activity of NF-ĸB (A) and PXR (B) in LS180 cells, after exposure for 2 h, 6 h, 24 h, or 30 h. Luminescence values of drug-treated samples were normalized to luminescence of untreated control samples. Data shown are the mean ± S.E.M. of three independent experiments with technical triplicates for each time point. Poly I:C (1 µg/mL, black squares with dotted line) served as a positive control for NF-ĸB activation (A), whereas rifampicin (20 µM, black diamonds with dotted line) was used as a positive control for PXR activation (without IFN-α 2a) (B). Statistical significance (see text and Table 1 for details) was evaluated using an unpaired, nonparametric, two-sided Mann–Whitney U-test comparing drug-treated samples with control samples of respective time point. Control data sets (poly I:C and rifampicin) were published before [35].