Table 1.
Phenotypic and genotypic characteristics of 20 positive cefotaxime-resistant E. coli, recovered from fecal and tracheal samples of a diarrheic chicken farm in Tunisia.
| Strains | Department | Chicken N° | Swabs | Profiles of Resistance to Non-β-Lactams | Genes Encoding Beta-Lactamases | blaCTX-M Genetic Environment | Gene Cassette Arrays in Class 1 Integrons | Other Resistance Genes Detected outside Integrons |
|---|---|---|---|---|---|---|---|---|
| EC1 | 1 | C1 | T | NAL, SUL, SXT, TET, S | blaCTX-M-15 + blaOXA-1 | ISEcp1/IS26-orf477 | dfrA17-aadA5 | tetA, sul2, sul3, aac(6′)-Ib-cr |
| EC2 | 1 | C1 | F | NAL, SUL, TET | blaCTX-M-15 + blaOXA-1 | ISEcp1/IS26-orf477 | ND | tetA, aac(6′)-Ib-cr, sul2 |
| EC3 | 5 | C5 | F | NAL, SUL, SXT, TET, S | blaCTX-M-15 + blaOXA-1 | ISEcp1/IS26-orf477 | dfrA17-aadA5 | tetA, aac(6′)-Ib-cr, sul2 |
| EC4 | 5 | C5 | T | NAL, SUL, SXT, TET, S, TOB, CN | blaCTX-M-15 +blaOXA-1 | Unknown-orf477 | dfrA17-aadA5 | tetA, aac(6′)-Ib-cr, sul2 |
| EC5 | 9 | C9 | T | NAL, CIP, TET, S | blaCTX-M-1 + blaTEM-1b | ISEcp1-orf477 | ND | tetB, aadA1, qnrB |
| EC6 | 3 | C3 | T | NAL, CIP, SUL, SXT, S | blaCTX-M-1 + blaTEM-1b | ISEcp1-orf477 | dfrA1-aadA1 | tetB, qnrB, sul2, sul3 |
| EC7 | 9 | C9 | F | SUL, TET | blaCTX-M-1 + blaTEM-1b | Unknown-orf477 | ND | tetA, sul2 |
| EC8 | 12 | C12 | F | NAL, CIP, SUL, SXT, TET, S | blaCTX-M-1 + blaTEM-1b | Unknown-orf477 | dfrA1-aadA1 | tetA, qnrB, sul3 |
| EC9 | 6 | C6 | T | NAL, SUL, SXT, TET, S, C | blaCTX-M-1 + blaTEM-1b | ISEcp1-orf477 | dfrA1-aadA1 | tetA, qnrB, sul2 |
| EC10 | 4 | C4 | F | SUL, TET, S, C | blaCTX-M-1 + blaTEM-1b | ISEcp1-orf477 | ND | tetB, sul2 |
| EC11 | 4 | C4 | T | SUL, TET | blaCTX-M-1 + blaTEM-1b | ISEcp1-orf477 | ND | tetB, sul2 |
| EC12 | 7 | C7 | F | TET, C | blaCTX-M-1 + blaTEM-1b | ISEcp1-orf477 | ND | tetB |
| EC13 | 8 | C8 | F | SUL, SXT, TET, S | blaCTX-M-15 + blaOXA-1 +blaTEM-1b | ISEcp1-orf477 | dfrA17-aadA5 | tetA, sul2 |
| EC 14 | 8 | C8 | T | NAL, CIP, SUL, SXT, S | blaCTX-M-15 + blaOXA1, blaTEM-1b | ISEcp1-orf477 | dfrA17-aadA5 | aac(6′)-Ib-cr, sul2 |
| EC15 | 6 | C6 | F | NAL, CIP, S | blaCTX-M-1 + blaTEM-1b | ISEcp1-orf477 | dfrA12-aadA2 | aac(6′)-Ib-cr |
| EC16 | 13 | C13 | F | SUL, SXT, S | bla CTX-M-1 | ISEcp1-orf477 | dfrA12-aadA2 | sul3 |
| EC17 | 2 | C2 | F | SUL, SXT, S | bla CTX-M-1 | ISEcp1-orf477 | dfrA17-aadA5 | |
| EC18 | 10 | C10 | F | SUL, SXT, TET, S | blaCMY-2 + blaTEM-1b | ISEcp1-unknown | ND | tetA, sul2, strA |
| EC19 | 10 | C10 | T | TET | blaCMY-2 + blaTEM-1b | ISEcp1-unknown | ND | tetA |
| EC20 | 11 | C11 | T | SUL, SXT, TET | blaCMY-2 + blaTEM-1b | ISEcp1-unknown | ND | tetA, sul2 |
EC: E. coli; F: Fecal swab; T: Tracheal swab; C: Chicken; NAL: Nalidixic acid; CIP: Ciprofloxacin; SUL: Sulfonamide; SXT: Trimethoprim/Sulfamethoxazole; TET: Tetracycline; C: chloramphenicol; S: Streptomycin; TOB: Tobramycin; CN: Gentamycin; ND: Integron non detected.