Figure 1.

In vitro HQ exposure enhances the viability of RAHFLS. RAHFLS (1 × 10⁴ cells) were incubated with culture medium or with HQ (1 or 10 µM) for 24 h, and had their viability and ability to proliferate determined through the MTT method (A) and the flow cytometry method based on the staining with an anti-CD90 antibody (B,C). Data represent mean ± SEM from three independent experiments and were analyzed by one-way ANOVA (A): ** p < 0.01 vs. culture medium; * p < 0.05 vs. HQ 1 µM; (B): * p < 0.05 vs. culture medium and vs. HQ 1 µM. (A): positive control: DMEM F12 culture medium supplemented with 10% FBS.