Figure 8.

Glycosaminoglycan-binding molecules surfen and protamine sulphate can inhibit wtS1S2 binding. RT4 cells were preincubated with the stated concentrations of surfen or protamine sulphate for 30 min at 37 °C, then with 100 nM wtS1S2 for a further 60 min at 37 °C. Bound wtS1S2 was detected using fluorescent secondary anti-His6, measured by flow cytometry. Data are shown as a percentage of the wtS1S2 attachment to untreated control cells. Data are the means ± SEM of 3–4 experiments performed in duplicate.