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Spike protein binding to heparin requires a polybasic furin cleavage site. Unfractionated heparin (UFH) and low-molecular-weight heparin, dalteparin (Dalt) were immobilised on 96-well plates and used to detect the binding of wtS1S2, mS1S2 lacking the furin cleavage site (mS1S2) or RBD using a biotinylated anti-His6 antibody and streptavidin-HRP. Data shown are the means ± SD from 2–3 separate experiments performed in duplicate. Tables provide the EC50 values for each protein.
