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. 2021 May 25;11(6):1545. doi: 10.3390/ani11061545

Table 3.

Primer abbreviations, full names, sequences, amplicon sizes (bp), annealing temperatures (°C), and accession number or reference.

Primer Abbreviation and Full Names Primer Sequences: Sense (S) and Anti-Sense (AS) Amplicon Sizes (bp) Annealing Temperatures (°C) Accession Number or References
REFERENCE GENES ACTB S: ACGGGCAGGTCATCACCATC 166 67 28
Beta actin AS: AGCACCGTGTTGGCGTAGAG
GADPH S: GGCATCGTGGAGGGACTTATG 186 62 28
Glyceraldehyde-3-phosphate dehydrogenase AS: GCCAGTGAGCTTCCCGTTGAG
CYTOKINES IL12p40 S: CACCAGCAGCTTCTTCATCA 105 60 33
Interleukin 12 subunit p40 AS: TACTCCCAGCTGACCTCCAC
IL4 S: GCCACACGTGCTTGAACAAA 63 60 30
Interleukin 4 AS: TCTCAACAGCTTGGCAAGCA
IL6 S: TAAGCGCATGGTCGACAAAA 150 60 32
Interleukin 6 AS: TTGAACCCAGATTGGAAGCAT
IL8 (CXCL8) S: AGAACTTCGATGCCAATGCAT 150 60 NM_173925
Interleukin 8 AS: GGGTTTAGGCAGACCTCGTTT
IL17A S: TCGTTAACCGGAGCACAAACT 120 60 32
Interleukin 17A AS: TGGCCTCCCAGATCACAGA
IL10 S: AGAACCACGGGCCTGACA 121 60 32
Interleukin 10 AS: ACCGCCTTGCTCTTGTTTTC
TGFß S: TGCTTCAGCTCCACAGAAAAGA 116 60 32
Transforming growth factor ß AS: AGGCAGAAATTGGCGTGGT
IFNɣ S: TTGAATGGCAGCTCTGAGAAAC 150 60 32
Interferon ɣ AS: TCTCTTCCGCTTTCTGAGGTTAGA
CHEMOKINES CXCL6 S: GAGAGCTGCGTTGTGTGTGT 107 60 29
Chemokine (C-X-C motif) ligand 6 AS: ACTTCCACCTTGGAGCACTG
CCL20 S: TTCGACTGCTGTCTCCGATA 172 62 28
Chemokine (C-C motif) ligand 20 AS: GCACAACTTGTTTCACCCACT
TRANSCRIPTION FACTORS FOXP3 S: TGGTGCAATCTCTGGAGCAA 116 60 30
Forkhead box P3 AS: GTCAGATGATGCCGCAGATG
GATA-3 S: CCAGACCAGAAACCGAAAAA 234 62 31
Trans-acting T-cell-specific transcription factor GATA-3 AS: ACCATACTGGAAGGGTGGTG
RORɣ (RORC gene) S: ACAGCCCTCGTCCTCATCAATGCC 145 60 30
RAR-related orphan receptor gamma AS: TGGGTGGCAGCTTTGCCAGGATA
TBX21 S: CGAGGACTATATACTGCCGC 133 61 31
AS: CAAGACCACGTCCACATACA