Figure 4.

GLUT-1 mediated the enhanced glycolysis in KFs. NFs and KFs were pretreated with or without 10 µM of WZB117 for 48 h and then incubated with base medium at 37 °C in a non-CO₂ incubator. Subsequently, 10 mM glucose, 1 µM oligomycin, and 75 mM 2-DG were injected to the medium. The ECARs were recorded and analyzed by the Seahorse XF-24 software, including (A) changes in the glycolytic function, (B) glycolysis, (C) glycolytic capacity, and (D) glycolytic reserve. Data are presented as mean ± SEM from 3 or more independent experiments. (N = 6, * p < 0.05, ** p < 0.01).