Table 1.

Mean DNA levels (calculated using normalization of C(t) value) of the S1, S2, and S3 samples between groups (single root or double roots).

Subgroup	S1	S2	S3	p-Value
Group1 (n = 20)	0.8927 ± 3.30 z	0.001 ± 0.00 cy	0.001 ± 0.02 y	<0.001
Group2 (n = 20)	0.8812 ± 1.95 z	0.006 ± 0.01 by	0.002 ± 0.00 y	<0.001
Control (n = 3, single rooted)	1.0 ± 1.72 z	0.013 ± 0.01 ay	0.001 ± 0.00 x	<0.001
p-value	0.99	<0.001	0.07	-
Group3 (n = 20)	1.0770 ± 4.52 z	0.003 ± 0.01 y	0.001 ± 0.00 bx	<0.001
Group4 (n = 20)	0.8985 ± 5.10 z	0.006 ± 0.10 y	0.002 ± 0.00 ax	<0.001
Control (n = 3, double rooted)	1 ± 1.91 z	0.005 ± 0.00 y	0.002 ± 0.00 aby	<0.001
p-value	0.98	0.504	<0.05	-

Different superscript letters (column: a~c, row: x~z) indicate statistically significant differences between groups (p-value [Multiple comparison] < 0.05); S1: before preparation; S2: just after preparation; S3: after incubation of 1 week.