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. Author manuscript; available in PMC: 2021 Jun 25.
Published in final edited form as: Cell Rep. 2021 Jun 1;35(9):109209. doi: 10.1016/j.celrep.2021.109209

Figure 3. Single-cell RNA-seq and evaluation of mTOR and AMPK activation in NK cells during MCMV infection.

Figure 3.

(A and B) Ncr1-Cox10Δ/Δ and Ncr1-WT NK cells were examined by single-cell sequencing 4 days after MCMV infection with uninfected controls.

(A) UMAP clustering of expression data using 20 principal components. Clusters 8 and 9 were expanded in response to MCMV infection in both groups, but cluster 10 was primarily expanded only with Cox10-deficient infected mice, accounting for ~20% of NK cells.

(B) Dot plot showing percentage of cells per sample expressing gene of interest (size) and average expression of gene (color) of transcriptionally regulated glycolytic genes upregulated during MCMV infection.

(C and D) NK cells from Ncr1-Cox10Δ/Δ and Ncr1-WT mice 4 days after MCMV expressing phosphorylated (C) mTOR and (D) AMPK in Ly49H− and Ly49H+ populations (mixed effects model with Tukey’s multiple comparison test, three independent experiments, n = 10–12 mice/group, pooled data, error bars represent SD). pAMPK was upregulated specifically in Ly49H+ Cox10-deficient NK cells.