. 2021 Jun 2;11(6):1652. doi: 10.3390/ani11061652

Table 1.

Caspase-3 activity in the granulosa cells isolated from preovulatory (F3-F1) follicles after 24 h of incubation with increased concentrations of 13 nm and 50 nm AgNPs.

Parameter	Control	Control + Buffer	Ag	Dose			SE	Group	Control vs. Control + Buffer	Ag	Dose	Ag × Dose	Dose (Trend)
Parameter	Control	Control + Buffer	Ag	0.1 µg/mL	1 µg/mL	5 µg/mL	SE	Group	Control vs. Control + Buffer	Ag	Dose	Ag × Dose	Linear	Quadratic
Caspase-3
F1	36.20 ^c	35.20 ^c	13 nm	44.20 ^c	76.80 ^ab	96.20 ^a	3.623	<0.001	0.756	<0.001	0.001	0.001	<0.001	0.456
			50 nm	49.60 ^c	44.20 ^c	52.00 ^bc							<0.005	0.219
F2	28.80 ^c	37.20 ^bc	13 nm	43.40 ^bc	73.60 ^a	53.80 ^ab	2.513	<0.001	0.113	0.001	0.019	0.095	0.123	0.015
			50 nm	45.00 ^abc	46.40 ^abc	35.40 ^bc
F3	25.60 ^de	33.20 ^cd	13 nm	41.20 ^abc	61.20 ^a	56.00 ^ab	2.456	<0.001	0.083	<0.001	0.003	<0.001	0.135	0.004
			50 nm	38.60 ^bcd	36.00 ^bcd	19.60 ^e							<0.001	0.638

^abcde means with different letters differ significantly (p ≤ 0.05).