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. 2021 Jun 8;10(6):1429. doi: 10.3390/cells10061429

Figure 6.

Figure 6

Chemotherapy increased the proportion of CD3+ TNFR2+ PD-1+ TILs at the expense of CD4+ TNFR2+ FOXP3+ and CD4+TNFR2+ PD-1+ TILs. Murine 4T1 TNBC cells were syngrafted to BALB/c mice in the presence of chemotherapy or a vehicle control; procedures and analyses were performed as described in Figure 3. (A) Ratio—set 1: ratio between CD8+ TNFR2+ PD-1+ TILs and CD4+ TNFR2+ FOXP3+ TILs. (A1) Ratio of CD8+ TNFR2+ PD-1+ TILs over CD4+ TNFR2+ FOXP3+ TILs, calculated on the basis of the percentages of the two subsets in each mouse. (A2) The ratio of CD8+ TNFR2+ PD-1+ TILs over CD4+ TNFR2+ FOXP3+ TILs, correlated with tumor volumes. (B) Ratio—set 2: ratio between CD8+ TNFR2+ PD-1+ TILs and CD4+ TNFR2+ PD-1+ TILs. (A1) Ratio of CD8+ TNFR2+ PD-1+ TILs over CD4+ TNFR2+ PD-1+ TILs, calculated on the basis of the percentages of the two subsets in each mouse. (A2) The ratio of CD8+ TNFR2+ PD-1+ TILs over CD4+ TNFR2+ PD-1+ TILs, correlated with tumor volumes. In the proportion and correlation analyses, the average value of the vehicle-treated group in each biological repeat was given the value of 1 and the values of all mice analyzed in that repeat were normalized accordingly. The value of each mouse is presented as a dot. *** p < 0.001 (two-tailed unpaired Student’s t-test; correlation analyses: Pearson; T.DIST).