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. 2021 Jun 3;13(6):830. doi: 10.3390/pharmaceutics13060830

Figure 2.

Figure 2

Effect of time and Cu2+ (50 μM), metformin (50 μM) and Cu2+/metformin (50 μM) on hIAPP (50 μM) aggregation in a 50 mM HEPES-buffered solution pH 7.4, at 25 °C. ThT (20 μM) was used as a fluorescence marker of the aggregation process.