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. 2021 May 28;14(6):521. doi: 10.3390/ph14060521

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibition of angiogenesis and VEGF levels by ATQ. Matrigel plug-bearing mice were fed with 50 mg/kg ATQ daily for 12 days. (a) Images of matrigels isolated from control and ATQ treated groups at Day 12. (b) Plugs were collected and analyzed for hemoglobin content by Drabkin’s reagent. (c) Average weight of matrigel plugs from control and ATQ treated mice. VEGF secretion was estimated by performing ELISA assay in (d) MDA-MB-231 and (e) HCC1806 cell supernatant treated with either DMSO control or varying concentrations of ATQ. (f) ELISA assay for VEGF in the tumor lysates obtained from control and ATQ treated mice group. Values were plotted as means ± SD. * Statistically significant as compared with control.