Figure 5.

Histological analysis shows reduced photoreceptor OS lengths in Myo1c-KO mice retinas: (A,B) Retinas from 2- and 6-month-old WT and Myo1c-KO mice were sectioned, using an ultra-microtome, and semi-thin plastic sections were obtained to evaluate pathological consequences of MYO1C loss. Quantification of OS lengths from H and E sections (C), two- month-old mice; (D), six-month-old mice) and ONL thickness (E), two-month-old mice; (F), six month-old mice, using “spider graph” morphometry. The OS lengths and total number of layers of nuclei in the ONL from H and E sections through the optic nerve (ON; 0 μm distance from optic nerve and starting point) were measured at 12 locations around the retina, six each in the superior and inferior hemispheres, each equally at 150 μm distances. RPE, retinal pigmented epithelium; OS, outer segments; IS, inner segments; ONL, outer nuclear layer; INL, inner nuclear layer; OPL, outer plexiform layer. Retinal sections (n = 5–7 sections per eye) from n = 8 mice for each genotype and time point (50:50 male-female ratio) were analysed. Two-way ANOVA with Bonferroni post-tests compared Myo1c-KO mice with WT in all segments. ** p < 0.005, for OS length in only 6-month-old Myo1c-KO mice, compared to WT mice; and n.s. (not significant) for ONL thickness in both 2-month and 6-month-old Myo1c-KO animals, compared to WT mice). (A,B) Scale bar = 100 μm.