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. 2021 May 31;10(6):680. doi: 10.3390/pathogens10060680

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Testing of POW-VLP immunogenicity by ELISA. (A) POW-VLPs induced IgG against POWV EDIII antigen after prime immunization. IgG titer increased after boost immunizations, showing 100% seroconversion in each dosage group (*** p < 0.0005). (B) POW-VLP elicited both IgG₁ and IgG₂ (A,B) antibody response after second boost immunization (*** p < 0.0005).