Fig. 3.

Circ3823 promotes CRC cell proliferation, migration, invasion and increases the tube junction forming ability of HUVEC in vitro. a Expression levels of circ3823 in HCT116 and SW480 cells treated with circ3823 plasmid and circ3823 siRNA. b, c, d, g, h Cell proliferation detection of CRC cells were measured by CCK-8, clone formation and EDU assay (magnification, × 200, scale bar, 50 μm). e 1 × 10⁴ SW480 cells were counted per experiment to determine apoptosis rate by flow cytometry via Annexin V-FITC/PI. f Apoptosis-related molecules were detected using qRT-PCR in SW480 cells. i, j, k, l, m Cell migration and invasion abilities were determined by transwell assays (magnification, × 100, scale bar, 100 μm). n, o Tube junction forming ability of HUVEC were determined by incubate with HCT116, SW480 cell culture supernatant after transfection (magnification, × 100, scale bar, 200 μm). Data were indicated as mean ± SD, ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001