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. 2021 Jun 7;11(6):530. doi: 10.3390/life11060530

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SS-1 inhibited the expression of costimulatory molecules and cytokines on spleen DCs. (A) The expression of CD80 and CD86 on spleen DCs was analyzed by flow cytometry. (B) The expression levels of CD80 and CD86 are presented as the mean fluorescence intensity (MFI). (C) IL-12, IL-23, and IL-4 mRNA was detected in purified CD11C+ DCs from the spleen using quantitative real-time PCR. The data were normalized to HPRT expression in each sample and the mean ± SEM (n = 5). * p < 0.05 and ** p < 0.01 versus SS-water group.