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. 2021 Jun 4;7(6):449. doi: 10.3390/jof7060449

Figure 1.

Figure 1

Penicillium species and antifungal proteins (AFPs) evaluated. (a) Representative images of growth on PDA plates of P. digitatum PHI-26 CECT 20796, P. italicum PHI-1 CECT 20909 and P. expansum CMP-1 CECT 20906 strains. (b) Amino acid sequence alignment of the tested AFPs. Phylogenetically different proteins are separated by a line. Conserved amino acids are represented with (*). Other conserved amino acids are represented with (.). Cysteine residues are shadowed in black. Amino acid alignment was performed using Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/ (accessed on 28 November 2020)). The number of amino acids of proteins is also shown at the end of the amino acid sequence. (c) Images of infected orange fruits with P. digitatum (green mold) and P. italicum (blue mold) and infected apple fruits with P. expansum (blue mold) at 7 days post-inoculation (dpi).