Figure 4.
Survival and weight changes in BALB/c mice with and without high-dose Cy immunosupression. Mice were vaccinated with TBE vaccine Tick-E-Vac and challenged with 100 LD50 of TBEV strain Vasilchenko. (a) Survival curves for vaccinated and control mice. (b) Weight changes after the virus challenge. Error bars represent SEM. Vac-Vac group was used as a control without Cy treatment (green); Cy-Vac-Vac received Cy treatment before the first vaccine dose (blue); Cy-Vac-Cy-Vac was treated with Cy twice: before the first and the second vaccine doses (red); Vac-Cy-Vac received Cy treatment before the second vaccine dose (black) and Vac was vaccinated only once (orange). N = 12–15 animals per group. Data only for mice surviving till 21 dpi was used for weight curves. * All brain samples from dead mice were considered RNA-positive. Statistical significance for mortality data was determined by log rank test (*** p < 0.001; **** p < 0.0001).