Figure 3.

Establishing the EndoScreen Chip. (a) Schematic workflow of the chip assay for JAC-C9 detection. JAC-mediated glycoprotein isolation from denatured serum samples and C9 labelling by SERS barcode-tagged anti-C9 antibody is performed under the stimulation of a nanoscopic fluid flow. JAC-C9 is detected by SERS mapping, where the Raman reporter DTNB that is conjugated to the SERS barcodes provides a characteristic Raman peak at 1335 cm⁻¹. (b) Raman spectra of 100 ng/mL C9 in PBS (black), 100 ng/mL C9 in diluted serum (pink), blank PBS (cyan) and diluted serum (purple). (c) Corresponding averaged Raman signal intensity of DTNB (1335 cm⁻¹). (d) Raman spectra and (e) averaged Raman signal intensity of DTNB (1335 cm⁻¹) obtained for designated C9 concentrations spiked in diluted serum. The error bars are the standard error of three replicates. ** p < 0.01 and *** p < 0.001.