Figure 5.

ATP synthase subunit beta (52 kDa), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (37 kDa) expression, and ratio GAPDH to ATP synthase subunit beta evaluated by Western blotting, from (A–C) infant MTX-treated (M), and control mice (C), and (D–F) adult mice exposed to a cumulative dose of 6.0 mg/kg MTX (M), and control (C) adult mice (experiment 2, infant and adult mice were euthanized 17 or 7 days after the last administration, respectively). Values are expressed as mean ± SD and were obtained from 6 (infant) and 6–7 (adult) animals from each treatment group. Statistical comparisons were made using the t-test: * p < 0.05, MTX 6.0 mg/kg vs. control. OD: optic density. Protein loading was confirmed by the Ponceau S staining (Figures S3 and S4).