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. 2021 May 26;14(6):510. doi: 10.3390/ph14060510

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Superoxide dismutase 2/ manganese-dependent superoxide dismutase (SOD2/MnSOD) (26.6 kDa), catalase (60 kDa) and glutathione peroxidase (22 kDa) expression evaluated by Western blotting, from (A–C) infant MTX-treated (M) and control (C) mice, and (D–F) adult mice exposed to a cumulative dose of 6.0 mg/kg MTX (M) and their respective controls (C) (experiment 2, infant and adult mice were euthanized 17 or 7 days after the last administration, respectively). Values are expressed as mean ± SD and were obtained from 6 (infant) and 6–7 (adult) animals from each treatment group. Statistical comparisons were made using the t-test: * p < 0.05, MTX 6.0 mg/kg vs. control. OD: optic density. Protein loading was confirmed by the Ponceau S staining (Figures S3 and S4).