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. 2021 May 26;14(6):510. doi: 10.3390/ph14060510

Figure 8.

Figure 8

Representative photomicrographs of heart immunohistochemistry from 6.0 mg/kg MTX-treated adult and respective controls (experiment 2, mice euthanized 7 days after the last administration), by a marker (CD68+) for macrophages M1 (A,B) and by a marker for (CD206) macrophage M2 (C,D) detection, and indicated by yellow arrows. Light micrograph (A,C) from the control of M1 (A) and M2 staining (C); light micrograph (B,D) from adult mice given a cumulative dose of 6.0 mg/kg MTX and performed staining for M1 (B) or M2 (D). Scale bar = 100 µm, n = 3. Images taken at 40× amplification.