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Representative photomicrographs of immunohistochemistry determination of NF-κB p65 in the cardiomyocytes-like cells from 6.0 mg/kg MTX-treated adult and respective controls (experiment 2, mice euthanized 7 days after the last administration) (A,B). Light micrograph (A) from the controls; and (B) from adult mice given a cumulative dose of 6.0 mg/kg MTX. Scale bar = 100 µm, n = 3. Images taken at 40× amplification.
