Figure 10.

Nuclear kappa B nuclear transcription factor (NF-κB) p52 (50 kDa) and NF-κB p65 (60 kDa) expression evaluated by Western blotting, from (A,B) infant MTX-treated (M) and control (C) mice, and (C,D) adult mice exposed to a cumulative dose of 6.0 mg/kg MTX (M) and respective controls (C) (experiment 2, infant and adult mice were euthanized 17 or 7 days after the last administration, respectively). Values are expressed as mean ± SD and were obtained from 5–6 (infant) and 6–7 (adult) animals from each treatment group. Statistical comparisons were made using the t-test: ** p < 0.01, MTX 6.0 mg/kg vs. control. OD: optic density. Protein loading was confirmed by the Ponceau S staining (Figures S3 and S4).