Table 4.

Number of cells staining positive for activated macrophages marked as M1 and macrophage marked as M2 in the heart of MTX-treated and control groups, in adult and infant animals.

	INFANT		ADULT
Immunohistochemistry
	Control	MTX 6.0 mg/kg	Control	MTX 6.0 mg/kg
M1 macrophage	4.28 ± 3.03	9.33 ± 7.70	12.83 ± 15.37	68.11 ± 22.77 ****
M2 macrophage	66.61 ± 43.40	62.56 ± 24.92	113.70 ± 23.10	96.56 ± 28.33

Results were expressed as mean ± SD. The value of macrophages was obtained from three animals from each treatment group. Statistical comparisons were made using the t-test for M2 macrophage evaluation in the adult group and the Mann–Whitney test for all infant group and M1 macrophage of adult group: **** p < 0.0001, MTX 6.0 mg/kg vs. control. Three animals per treatment, and for each animal, six photos of different areas of cardiac tissue were evaluated and quantified using ImageJ software version 1.52a.