Table 5.

Number of cells staining positive for activated NF-κB in the heart of MTX-treated and control groups, in adult and infant animals.

	INFANT		ADULT
Immunohistochemistry
	Control	MTX 6.0 mg/kg	Control	MTX 6.0 mg/kg
NF-ĸB p65 cells	28.00 ± 12.35	26.00 ± 8.62	56.28 ± 15.69	75.17 ± 17.05 **

Results were expressed in number of cells positive to NF-κB p65 and as mean ± SD. Results were obtained from three animals from each treatment group. Statistical comparisons were made using the t-test for adult group and the Mann–Whitney test for infant group: ** p < 0.01, MTX 6.0 mg/kg vs. control. Three animals per treatment, and for each animal, six photos of different areas of cardiac tissue were evaluated and quantified using ImageJ software version 1.52a.