Figure 2.

Immunohistochemistry of retinal whole-mount micrographs of mice eyes. The A,B retinas were labeled with anti-Iba-1 (microglia), showing the comparison of Iba-1 + microglia in the OHT untreated (A) and treated with saffron extract (B) eyes in the outer plexiform layer of the retina. It has been noted that in the OHT mice eyes treated with saffron extracts, less activation and fewer microglial cells were observed than in the untreated eyes. The C,D retinas were stained with anti Brn3a (RGCs). The C,D comparative micrographs showed the Brn3a + RGCs in the untreated (C) and treated with saffron extract (D) mice retinas. It was also detected a higher RGCs density in the treated versus the untreated rat retinas. RGC: retinal ganglion cells.