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. 2021 Jun 4;11(6):837. doi: 10.3390/biom11060837

Figure 2.

Figure 2

Expression of cytokines/chemokines and TLRs in M1 and M2 macrophages obtained from CFA mice. (A) ELISA of cytokines (IL-1β, IL-6, IL-12, TNF-α, IL-10) and chemokines (CCL2, CCL3, CCL4, CCL22, CXCL9, and CXCL10) in supernatants of M1 or M2 macrophages. Data are reported as pg/mL and represent 10 independent experiments means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. (B,C) qRT-PCR analysis of the expression of both bacterial (TLR1, TLR2, TLR4, TLR5, TLR6) (B) and viral (TLR3, TLR7, TLR8, TLR9) (C) Toll-like receptors in M1 and M2 macrophages. Cycling threshold values are normalized to those of mRNA encoding β2-microglobulin and data are normalized to the maximum value obtained for each donor. Data are expressed as arbitrary units (A.U.) and are shown as means ± SEM of eight independent experiments each in duplicate. * p < 0.05.