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. 2021 Jun 7;7(6):454. doi: 10.3390/jof7060454

Figure 5.

Figure 5

Phagolysosome formation is critical to prevent A. fumigatus growth within airway epithelial cells. (A) Changes in the number of lysosomes per cell in 16HBE and A549 lung epithelial cells challenged and unchallenged with A. fumigatus spores. Asterisks represent differences between spores or germlings compared with uninfected for each cell line. Lines represent differences between groups for each cell line. (B) Intensity of relative LAMP1-GFP fluorescence intensity around spore or germlings-containing phagolysosomes of A549 and 16HBE lung epithelial cells. Asterisks represent differences between spores and gemlings for each cell line. (C) Fate of A. fumigatus spore within phagosomes (PS) and phagolysosomes (PLS) of A549 and 16HBE lung epithelial cells. Asterisks represent differences for each morphotype between cell lines. Data represents mean and standard deviation of three biological replicates assayed in technical triplicates. (** p < 0.01; *** p < 0.001; **** p < 0.0001).