Figure 2.

Effect of 7β-OHC on cell viability and proliferation. (A) MTT assay: Cells were incubated with different concentrations of 7β-OHC (10–60 µg/mL) for 24 h. (B) MTT assay: Cells were incubated with different concentrations of H₂O₂ (50–150 µM) for 24 h. Data are represented as mean ± SD. 7β-OHC: 7β-hydroxycholesterol; H₂O₂: Hydrogen peroxide. Statistical analyses were performed using the Mann–Whitney test or student t test. * Statistical differences were significant between the control and 7β-OHC or H₂O₂-treated cells (p < 0.05).