Table 3.
Levels of enzymatic and non-enzymatic antioxidants under pre-treatment with NSO or MTSO.
| SOD (% Control) | GPx (% Control) | Thiols (µmol/mg of Protein) |
|
|---|---|---|---|
| Control | 100 ± 0.00 | 100 ± 0.00 | 0.95 ± 0.01 |
| (EtOH 0.2%) | 97.85 ± 0.76 | 91.18 ± 9.36 | 0.92 ± 0.14 |
| (DMSO 0.4%) | 98.17 ± 2.34 | 98.25 ± 4.18 | 0.97 ± 0.09 |
| (EtOH + DMSO) | 97.88 ± 1.50 | 99.18 ± 9.28 | 0.95 ± 0.01 |
| NSO (100 µg/mL) | 99.30 ± 4.18 | 97.41 ± 5.40 | 0.94 ± 0.02 |
| MTSO (100 µg/mL) | 101.47 ± 2.78 | 96.81 ± 1.14 | 0.98 ± 0.07 |
| 7β-OHC (40 µg/mL) | 34.73 ± 1.43 * | 56.42 ± 5.25 * | 0.48 ± 0.08 * |
| 7β-OHC + NSO (100 µg/mL) | 47.77 ± 0.89 # | 75.65 ± 5.10 | 0.45 ± 0.08 |
| 7β-OHC + MTSO (100 µg/mL) | 109.63 ± 7.73 # | 106.26 ± 10.30 # | 0.59 ± 0.04 |
Data are represented as mean ± SD. 7β-OHC: 7β-hydroxycholesterol; SOD: superoxide dismutase; GPx: glutathione peroxidase; NSO: Nigella sativa seed oil; MTSO: Milk Thistle seed oil; DMSO: dimethyl sulfoxide; EtOH: Ethanol. Statistical analyses were performed using the Mann–Whitney test or student t test. * Statistical differences were significant between the control and vehicle (EtOH) or 7β-OHC-treated cells (p < 0.05). # Statistical differences were significant between 7β-OHC-treated cells and 7β-OHC + (NSO or MTSO) -treated cells (p < 0.05).