Table 4.
Levels of lipid peroxidation biomarkers under pre-treatment with NSO or MTSO.
| MDA (µmol/mg of Protein) |
CD (µmol/mg of Protein) |
|
|---|---|---|
| Control | 68.09 ± 3.43 | 0.55 ± 0.83 |
| (EtOH 0.2%) | 70.05 ± 5.70 | 0.40 ± 0.10 |
| (DMSO 0.4%) | 73.43 ± 1.11 | 0.54 ± 0.04 |
| (EtOH + DMSO) | 71.32 ± 0.43 | 0.56 ± 0.04 |
| NSO (100 µg/mL) | 69.96 ± 2.60 | 0.59 ± 0.03 |
| MTSO (100 µg/mL) | 70.93 ± 2.38 | 0.44 ± 0.01 |
| 7β-OHC (40 µg/mL) | 99.39 ± 2.50 * | 0.89 ± 0.07 * |
| 7β-OHC + NSO (100 µg/mL) | 79.64 ± 1.60 # | 0.52 ± 0.12 # |
| 7β-OHC + MTSO (100 µg/mL) | 75.02 ± 0.90 # | 0.68 ± 0.19 |
Data are the mean ± SD. 7β-OHC: 7β-hydroxycholesterol; MDA: malondialdehyde; CDs: conjugated dienes; NSO: Nigella sativa seed oil; MTSO: Milk Thistle seed oil; DMSO: dimethyl sulfoxide; EtOH: Ethanol. Statistical analyses were performed using the Mann–Whitney test or student t test. * Statistical differences were significant between the control and vehicle (EtOH) or 7β-OHC-treated cells (p < 0.05). # Statistical differences were significant between 7β-OHC-treated cells and 7β-OHC + (NSO or MTSO) -treated cells (p < 0.05).