Figure 3.

Conserved regions of ERD14 contribute to cell protection. (A) Schematic representation of the conserved regions within ERD14. (B) Survival rate (viability) is reduced by the applied heat stress to 26.2% (compared to non-stressed cells) for non-transformed cells, 36.8% for cells containing an empty vector, and about 39% in the case of overexpression of control proteins: GST (38.4%) and IDP calpastatin (38.7%). Overexpression of ERD14 increases the survival rate to 74.5%. Removing the conserved regions by randomizing the amino acid sequence (Full-Scr) abolished the protective effect (viability 38.9%). (C) Protective effect of a series of mutants in which conserved regions of the protein have been individually deleted. Data represent mean ± SEM and the results of at least 18 parallels for each construct. Significant differences (p < 0.05) compared to WT ERD14 are labeled with asterisks (*).