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. 2021 Jun 8;22(12):6190. doi: 10.3390/ijms22126190

Figure 5.

Figure 5

Interplay of different conserved elements in ERD14. (A) Protective effect of double- and triple deletion mutants, in which two or three segments are deleted (K-segments Ka, Kb, and Kc, as well as Chp-, S-, and H-segments; cf. sequence: Supplementary Table S1). Data represent mean ± SEM and the results of at least 18 parallels for each construct. Significant differences (p < 0.05) compared to WT ERD14 are labeled with asterisks (*). (B) Protective effect of scrambled mutants, in which either the full sequence is scrambled (Full-Scr), or particular binding regions are kept intact (e.g., Kc in Scr-Kc) and the rest of the sequence is randomized. Data represent mean ± SEM and the results of at least 18 parallels for each construct. Significant differences (p < 0.05) compared to WT ERD14 are labeled with asterisks (*). (C) The relative % contributions of motifs of ERD14 (Ka, S, Chp, Kb, H, and Kc) and their combinations (e.g., KcS is combined Kc and S) are quantified, as given in Materials and Methods (Supplementary Table S2). Their % contributions to the activity above the given background, WT ERD14 and Full-Scr ERD14, either alone or in combinations, are presented in a matrix. Numbers highlighted in color demonstrate that effects are usually higher in the WT than in the scrambled background.