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. 2021 Jun 7;11(6):754. doi: 10.3390/brainsci11060754

Figure 1.

Figure 1

CSMN that are vulnerable in ALS due to different underlying causes display reduced levels of 5-methylcytosine (5mC). (A) Representative image of layer V of motor cortex from WT-UeGFP mouse at P120 showing GFP labelled CSMN and 5mC expression. Box enlarged (A′,A″, arrow heads). (B) Representative image of layer V of motor cortex from hSOD1G93A-UeGFP mouse at P120 showing GFP labelled CSMN and 5mC expression. Box enlarged (B′,B″, arrow heads). (C) Quantitative analysis of average percentage of CSMN expressing 5mC in hSOD1G93A-UeGFP mouse at P120. (D) Quantitative analysis of average intensity of 5mC expression in CSMN of hSOD1G93A-UeGFP mouse at P120. (E) Representative image of layer V of motor cortex from WT-UeGFP mouse at P150 showing GFP labelled CSMN and 5mC expression. Box enlarged (E′,E″, arrow heads). (F) Representative image of layer V of motor cortex from prp-TDP-43A315T-UeGFP mouse at P150 showing GFP labelled CSMN and 5mC expression. Box enlarged (F′,F″, arrow heads). (G) Quantitative analysis of average percentage of CSMN expressing 5mC in prp-TDP-43A315T-UeGFP mouse at P150. (H) Quantitative analysis of average intensity of 5mC expression in CSMN of prp-TDP-43A315T-UeGFP mouse at P150. ** p < 0.001, *** p < 0.0005; Scale bar: 20 µm.