Table 1.

Comparison of HHP, LTLT, HTST and UV-C expressed in percentage of raw Human Milk. HHP preserve Biocomponent of HM: Lipase, lactoferrin, lysozyme and IgAs, but Demazeau HHP is the lower pressure with a maximum of 350 MPa and four cycles, one between 50–150 MPa to allow the germination of Bacillus cereus Spores and the last cycle at 350 MPa destroyed the vegetative form; Pitino considered that <10³ cfu is normal, but we consider that only <1 cfu/mL is sterile. Also, he did not do a challenge test with more than 10⁶ of Bacillus cereus Spores, and we say that with only one cycle, we can destroy it, even with one cycle of HHP > 600 mPa.

	Demazeau HHP	Buffin (LTLT)	MORO (HTST)	Christen (UV-C)	Pitino (HHP)
HHP	yes	no	no	no	yes
max MPa	350 × 15′				500 × 8′
Temperature	38 °C	62.5 °C × 30′	72 °C × 5–15′’		4 °C
UV-C	no	no	no	yes
Lipase	79–100%	0	0	52%	80–100%
Lactoferrin	100%	21%	25%	40%	70%
Lysozyme	95%	84%	66%	30%	100%
IGas	64%	71%	nd	nd	nd
Bacteriology: cfu/mL	<1 cfu/mL Sterile	<1 cfu/mL sterile	<1 cfu/mL sterile		<103 cfu/mL No sterile
Bacillus cereus	Destroyed	No Destroyed	No Destroyed	No Destroyed	No Destroyed