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. 2021 Jun 7;10(6):505. doi: 10.3390/biology10060505

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Gut and skin mucosal morphology of barramundi fed partially defatted BSF larvae protein and oil as FM protein and oil replacement diets. (a) Schematic representation of histometric measurement of muscular layer thickness (Mlt), fold height (Fh), fold width (Fw), and acidic mucins (Am) of distal gut (PAS, 40× magnification, scale bar 50 µm). (b) Quantification of Mlt, Fh, Fw, and Sam of the distal gut of barramundi. (c,d) Skin histometric measurement and quantification of skin muscular layer thickness (Mlt) and number of skin acidic mucin (Am) (Alcian Blue pH 2.5 stain, 40× magnification). Data were expressed as mean ± SE, n = 6. Bar with p-value denotes significant level among the experimental treatments (one-way ANOVA; Tukey post-hoc test; significant at p > 0.05).