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. 2021 May 28;10(6):1226. doi: 10.3390/foods10061226

Table 5.

Life cycle analyses (LCA) of alternative proteins.

Protein Type Energy Use
(MJ/kg)
GHG Emission
(kg CO2-eq/kg Product)
Water Use or Eutrophication a Land Use
(m2a/kg) b
Reference
Cultured meat
Minced beef 1 26–33 1.90–2.24 0.36–0.52 m3/kg meat (W) 0.19–0.23 [28]
CHO 2 106 7.5 7.9 g PO4-eq/kg meat (E) 5.5 [205]
Plant-based meat
Beyond Burger® 54.15 3.35 28.84 m3/kg meat (W) 3.97 [209]
Impossible Burger® NA 3.5 0.11 m3/kg meat (W); 1.3 g PO4-eq/kg meat 2.5 [210]
Insect protein
Mealworm (T. molitor and Zophobas morio) 33.68 2.65 NA 3.56 [206]
Black soldier fly (H. illucens) 21.20–99.60 1.36–15.10 NA 0.032–7.03 [207]
Cricket (G. bimaculatans and A. domesticus) NA 2.29 0.43 m3/kg cricket (W); 0.00047 kg P-eq and 0.020 kg N-eq/kg cricket (E) NA [208]
Single-cell protein
Spirulina tablets (A. platensis) 7.88–12.7 5.05–7.71 0.015–0.022 kg N-eq/kg tablet (E) NA [211]
Micoalgal protein (A. platensis) 1225.6–3338.3 78.1–196.3 3.2–3.3 m3/kg protein meal (W); 49.2–85.3 kg N-eq/kg protein meal (E) 1.7–4.3 [212]
Microalgal protein (C. vulgaris) 217.1–4181.3 14.7–245.1 0.3–3.9 m3/kg protein meal (W); 40.6–105.3 kg N-eq/kg protein meal (E) 1.9–5.4 [212]
Mycoprotein 60.07–76.8 5.55–6.15 NA 0.79–0.84 [213]
Bacterial protein (Cupriavidus necator) NA 0.81–1 0.0001–0.0038 m3/kg protein (W); 0.000333 kg P-eq/kg protein (E) 0.029–0.085 [214]
Bacterial protein (hydrogen-oxidizing bacteria) 200 8 2.5 m3/kg protein (W);
0.0025 kg P-eq/kg protein and 0.00035 N-eq/kg protein (E)
0.8 [215]

GHG, greenhouse gas; NA, not available. a Water use (W) is expressed in volumetric unit (m3 or L/weight of product), whereas eutrophication (E) is a measure of the amount of contaminants released into freshwater or marine environments (g contaminant/weight of product). b Land use is expressed in annual area occupation (m2a). 1 Cyanobacterial hydrolysate was assumed as the growth medium. 2 LCA was conducted based on the proliferation of Chinese hamster ovary (CHO) in a growth medium mainly comprising basal medium and soy hydrolysate.