Figure 1.

A schematic representation of our strategy. (A) State-of-the-art synergies. A 3D cellular angiogenesis assay that mimics human adult angiogenesis in vivo as well as the first stages of the angiogenic cascade was coupled to state-of-the-art technologies (LC-MS and NMR spectroscopy) and chemo-informatics tools/databases to assess the anti-angiogenic action of amifostine in a human adult angiogenesis setting. (B) Modular analysis (n = 3 cell lines, n = 2 cell culture conditions, n = 32 test conditions, n = 3 biological replicates (per test condition), and n = 3 technical replicates (per test condition)). Group comparisons are color-coded; orange, angiogenesis stimulation and blue, angiogenesis inhibition. A total of n = 240 metabolites were identified and quantified by LC-MS-based analysis, whereas 40 metabolites were identified and quantified from the 1H CPMG NMR spectra. A total of 36 metabolites were identified and quantified by both LC-MS and NMR spectroscopy.