. 2021 Jun 1;13(6):394. doi: 10.3390/toxins13060394

Table 2.

LC–MS/MS detection parameters of CPs.

Cyanopeptide	t_R (min)	Precursor Ion	Product Ions	Collision Energy (eV)	Product Ion Assignment	Ref.
MG FR1	18.9	728.0 [M+H]⁺	100.0	40	MeLeu immonium ion	[46]
			128.2 ^Q	40	Ahda fragment (C₈H₁₈N)	[45]
			384.2	40	[M + H-Tyr-Tyr]⁺	[46]
MG FR3	15.6	728.0 [Μ+H]⁺	128.2	40	Ahda fragment (C₈H₁₈N)	[45]
			233.0 ^Q	40	[Pro-Tyr-CO + H]⁺	[45]
			442.0	40	[Pro-Tyr-Tyr + H]⁺	[46]
MG T1	15.5	732.0 [Μ+H]⁺	162.1	40	Cl-Ahda fragment (C₈H₁₇NCl)	[44]
			233.0 ^Q	40	[Pro-Tyr-CO + H]⁺	[45]
			442.2	40	[Pro-Tyr-Tyr + H]⁺	[46]
MG T2	15.7	698.0 [M+H]⁺	128.2	40	Ahda fragment (C₈H₁₈N)	[45]
			233.0 ^Q	40	[Pro-Tyr-CO + H]⁺	[45]
			442.2	40	[Pro-Tyr-Tyr + H]⁺	[46]
AER 602/Κ139	13.8	603.2 [Μ+H]⁺	122.0	40	[Choi immonium-H₂O]⁺	[47]
			140.0	40	Choi immonium ion	[47]
			221.2 ^Q	40	Leu-Choi fragment	[45]
AER 298A	13.6	605.3 [Μ+H]⁺	122.0	40	[Choi immonium-H₂O]⁺	[47]
			140.0	40	Choi immonium ion	[47]
			311.0 ^Q	40	[Choi-Argininol-NH₂ + H]⁺	[48]
AEG A	24.6	561.4 [Μ+H]⁺	86.0	40	[PreNH₃]⁺	This study
			112.0	40	TzlCO	[20]
			154.2 ^Q	40	[(Pre)₂NH₂]⁺	This study
AP B	14.8	837.4 [Μ+H]⁺	84.0	40	Lys immonium ion	[49]
			201.1 ^Q	40	CO-Arg (side chain)	[49]
			637.3	40	[Lys-Phe-MeAla-HTyr-Val + 2H]⁺	[49]
AP F	15.2	851.3 [Μ+H]⁺	84.0	40	Lys immonium ion	[49]
			201.0 ^Q	40	CO-Arg (side chain)	[49]
			651.4	40	[Lys-Phe-MeAla-HTyr-Ile + 2H]⁺	[49]
OSC Y	19.9	858.4 [Μ+H]⁺	84.0	40	Lys immonium ion	[50]
			405.0 ^Q	40	[M + H-Tyr-(Htyr-Ile)]⁺	[50]
			681.4	40	[M + H-Htyr]⁺	[50]

^Q quantifier ion.