Table 2.
Effects of a 3D-IVM system in alginate-engineered microbeads on the developmental competence of prepubertal lamb oocytes.
Culture Condition |
No. of Inseminated Oocytes |
No. of Zygotes Evaluated after IVF |
Embryo Developmental Stages Number (%) |
Total Cleavage Number (%) |
|||||
---|---|---|---|---|---|---|---|---|---|
2- to 3- Cell | 4- to 7- Cell | 8- to 15- Cell | 16- to 31- Cell | Morula (>32 Cells) |
Blastocyst (>64 Cells) |
||||
2D | 163 | 157 | 4 (2.5) a |
22 (14.0) |
37 (23.6) |
19 (12.1) |
4 (2.5) |
5 (3.2) |
91 (57.9) a |
3D | 175 | 172 | 25 (14.5) c |
34 (19.8) |
30 (17.5) |
16 (9.3) |
4 (2.3) |
9 (5.2) |
118 (68.6) b |
Chi-squared test: within each column, different superscripts indicate statistically significant differences; a,b: p < 0.05; a,c: p < 0.001.