Figure 3.

The IC-pPDXC-63 cell line is biphenotypic in vitro and resumes expression of known specific cellular markers and transcription regulators of the NOR and MES identities. UMAP representation of the single-cell RNA-sequencing of the IC-pPDXC-63 model, with each dot corresponding to a cell [29]. (A) The TH and DBH enzymes as well as the neuroendocrine secretory protein CHGA are expressed in noradrenergic cells, while CD44, COL1A1, and FN1 are specifically expressed in cells of mesenchymal identity. (B) The transcriptional regulators PHOX2B, GATA3, and MYCN are specifically expressed in noradrenergic cells, while RUNX2, WWTR1, and IRF1 exhibit the opposite expression pattern.