Table 2.
Parameter values for orthogonal partial least squares-discriminant analysis (OPLS-DA) models.
| OPLS-DA Model | R2 | Q2 | p Value |
|---|---|---|---|
| IBS vs. Healthy | 0.96 | 0.79 | <0.001 |
| IBS vs. Healthy (LASSO) † | 0.86 | 0.78 | <0.001 |
| IBS-C vs. IBS-D | 0.99 | 0.56 | 0.04 |
| IBS-C vs. IBS-nonCnonD | 0.46 | 0.14 | 0.13 |
| IBS-D vs. IBS-nonCnonD ‡ | 0.75 | −0.26 | >0.99 |
| IBS mild vs. IBS moderate § | 0.38 | −0.005 | >0.99 |
| IBS mild vs. IBS severe ‡,§ | 0.84 | −0.02 | >0.99 |
| IBS moderate vs. severe § | 0.42 | 0.05 | 0.52 |
| IBS with anxiety vs. IBS without anxiety ¶ | 0.72 | 0.12 | 0.51 |
Note: All OPLS-DA models are based on the intestinal microenvironment profile, including 54 microbial taxa and 155 metabolites analysed, unless specified. R2 value indicates goodness of the fit of the model; Q2 value describes the prediction ability of the model. The p value depicts statistical significance based on ANOVA of the cross-validated residuals (CV-ANOVA). Abbreviations: IBS-C, IBS with constipation; IBS-D, IBS with diarrhoea; IBS-nonCnonD, mixed-type IBS and unclassified IBS. † IBS vs. healthy (LASSO) model based on 21 selected variables (n = 5 bacterial taxa and n = 16 metabolites). ‡ OPLS-DA model calculated by adding two first forced components. § Based on IBS-SSS, IBS Severity Scoring System. ¶ Based on Hospital Anxiety and Depression Scale.