Results of NMR studies of the structure of unmodified and succinylated forms of BacSp222. Panel (A) illustrates the superimposition of the most representative structures of unmodified BacSp222 (cyan) and suc-K20-BacSp222 (red). The side chain of lysine 20 in both structures is represented as sticks–hydrogens white, nitrogens blue, carbons of unmodified lysine cyan, and carbons of modified lysine orange. The superimposition of 1H-15N HSQC spectra and 1H-13C HSQC spectra tuned to aromatic carbons for unmodified BacSP222 (green) and suc-K20-BacSp222 (red) is presented in panels (B),(C), respectively.