Figure 1.

Single-cell analysis of 10-week-old BL6 mouse knee joints. (A) Graphical representation of the experimental workflow. Cartilage from mouse knee joints was dissected, dissociated into single cells, and subjected to immune and blood cell depletion. Viable cells from the remaining fraction were sequenced. (B) Cell clusters from scRNA-seq analysis visualized by Uniform Manifold Approximation and Projection (UMAP). Colors indicate clusters of various cell types. (C) Feature plot showing the expression of chondrocyte marker Acan. (D) Dot plot showing the expression of selected markers of various cell types. Dot size represents the % of cells expressing a specific marker, while the intensity of color indicates the average expression level for that gene, in that cluster. (E) Violin plot showing the expression of key transcription factors enriched in chondrocytes.