Figure 1.

Fragments of dsDNA containing TP53 c.818G > A and KRAS c.35G > T mutations were found in SW480-derived exosomes. Exosomal DNA was extracted from vesicles produced by untreated (ExoDNA CTRL) or lipopolysaccharides (LPS) stimulated (ExoDNA LPS) cells and analyzed using Qubit (A) and Bioanalyzer (B). The mutated copy analysis of TP53 (C) and KRAS (D) was conducted using droplet digital PCR, and representative results are reported (intensity plot). NTC (negative control). Data are shown as mean (n = 6) ± SD. * p < 0.05, compared with the exosomes produced by untreated cells.